Selective depletion of donor alloreactive T cells by using immuno-magnetic cell sorting / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 637-640, 2005.
Article
在 Zh
| WPRIM
| ID: wpr-356498
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore a new method of alleviating graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation through selective elimination of human alloreactive T cells expressing either CD25(+) or CD69(+) by immuno-magnetic cell sorting (MACS). Healthy donor peripheral blood mononuclear cells were cocultivated with bone marrow mononuclear cells from HLA-nonidentical leukemia recipient with remission in one-way mixed lymphocyte culture (OWMLC). After 3 days, both CD25(+) and CD69(+) lymphocytes were removed by MACS. The depleted donor fraction and untreated donor cells were then rechallenged in a secondary mixed lymphocyte culture (MLC) with the original stimulator cells or a third party to assess relative alloreactivity. The results showed that 50% inhibition of the secondary MLC was observed in the depleted donor fraction. Alloreactivity against unrelated third-party cells was largely preserved. It is concluded that this method reduces alloreactivity while retaining reactivity against a third party target in vitro.
全文:
1
索引:
WPRIM
主要主题:
T-Lymphocytes
/
Antigens, Differentiation, T-Lymphocyte
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Antigens, CD
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Bone Marrow Transplantation
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Immunomagnetic Separation
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Cell Biology
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Lectins, C-Type
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Allergy and Immunology
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Interleukin-2 Receptor alpha Subunit
/
Flow Cytometry
限制:
Humans
语言:
Zh
期刊:
Journal of Experimental Hematology
年:
2005
类型:
Article