ABSTRACT
Objective:
To investigate the expression of Oct4 and Wnt2 in
human glioma tissues and its relationship with the clinicopathological features of
glioma.
Methods:
Fifty-six
paraffin blocks were obtained from
glioma patients receiving
surgery. The
diagnosis of these
patients were confirmed by
pathology in our
hospital from 2006-2009. Immunohistochemi-cal
staining was used to examine Oct4 and Wnt2 expression in the
brain tissues of 10
patients with
acute brain injury and 56
glioma tissues (including 15 recurrent cases).
Results:
The normal
brain tissues were negative of Oct4, with only one case showing weak Wnt2 expression. Thirty-four of the 56
glioma tissues showed positive expression of Oct4 (60.7%), and 40 showed positive expression of Wnt2 (71.4%). Positive expression rates of Oct4 and Wnt2 in low-grade and high-grade
glioma tissues were 46.2 %, 73.3% and 57.7 %, 83.3%, respectively (P < 0.05). Oct4 positive rates in the
recrudescence and newly diagnosed
glioma tissues were 86.7% and 51.2%, respectively (P < 0.05). Oct4 expression in the
glioma tissues was positively correlated with that of Wnt2 (r = 0.537, P < 0.01).
Conclusion:
Expression of Oct4 and Wnt2 is associated with the malignant degrees of
glioma, and Oct4 expression is related to the
recurrence of
glioma. Oct4 might participate in the development and progression of
brain glioma through
Wnt signaling pathway.