ABSTRACT
BACKGROUND:
Mesenchymal stem cells (MSCs) are capable of differentiating into a variety of lineages, including
bone,
cartilage, or fat, depending on the inducing stimuli, specific
growth and differentiation factors.Recombinant
human bone morphogenetic protein 2 (rhBMP-2) produced by
gene engineering has an obvious osteoinductive activity and can induce undifferentiated mesenchymal
cells into
cartilage and
bone irreversibly, resulting in new
bone formation.
Basic fibroblast growth factor (bFGF) modulates chondrogenic and osteogenic differentiation of MSCs.
OBJECTIVE:
To investigate the effect of bFGF and rhBMP-2 on the differentiation and proliferation of cultured
rabbit mesenchymal stem cell in order to find out an optimal way of
osteogenesis instead of conventional osteogenic supplements (OS).
DESIGN:
A randomized and controlled trial.
SETTING:
General Institute of Orthopaedic Oncology, Tangdu
Hospital,Fourth
Military Medical
University of
Chinese PLA.MATERIALS The subjects were
rabbit mesenchymal stem cells cultured by the author.
METHODS:
This experiment was conducted at the Center of Orthopaedic
Surgery, General Institute of Orthopaedic Oncology, Tangdu
Hospital,Fourth
Military Medical
University of
Chinese PLA from June 2004 to December 2004. ①
Rabbit MSCs cultured
in vitro were treated with different
growth factor (100 μg/L rhBMP-2, 100 μg/L bFGF, 10 μg/L rhBMP-2and 100 μg/L bFGF and OS; ②The proliferation and differentiation of MSCs were observed through activity of MTT, expression of
alkaline phosphatase(ALP), and von Kossa
staining.MAIN OUTCOME
MEASURES:
the rate of proliferation and the activity of ALP.
RESULTS:
①rhBMP-2 could promote the proliferation and differentiation of MSCs, especially the
cell differentiation; ②bFGF could stimulate the proliferation , the
cellular proliferation rate increased 100% as compared with
control group, and has no effect on differentiation of MSCs , but it could enhance effect on the
cell proliferation of rhBMP-2.
CONCLUSION:
bFGF and rhBMP-2 are effective induction factors for MSCs. Both of them can stimulate the proliferation and differentiation of MSCs
in vitro. bFGF and rhBMP-2 exerted a synergetic action in
speeding up the pace of osteoinduction and
osteogenesis and can be used to differentiate
seed cells for
tissue engineering bone.