ABSTRACT
Objective:
To detect the correlation of
lymphatic vessel density (LVD) with a high mobility group box-1
Protein (
HMGB1) and
tumor-associated macrophages (TAMs) in cervical
carcinoma and the effect on
prognosis.
Methods:
Immunohistochem-istry was applied to detect
HMGB1, CD68, and D2-40 expressions in cervical
squamous cell carcinoma in 93 cases. t test,χ2 test, Spear-man rank correlation
analysis, Kaplan-Meier method, and Cox regression were performed to analyze the expression levels, correlation, and
prognosis.
Results:
HMGB1 protein, CD68, and D2-40 were highly expressed in CSCC. As
HMGB1 and TAM expressions in-creased,
lymphatic vessel density increased. As
HMGB1 and TAM expressions decreased,
lymphatic vessel density decreased. Positive correlations were also found between the
HMGB1 protein, TAM content, and LVD. In the group with low
HMGB1 and TAM expres-sions, the
survival time of the group with a high LVD expression was significantly lower than that of the group with a low LVD expres-sion. A multivariate Cox regression model showed that
HMGB1 and
lymph node metastasis were independent
prognostic factors. TAMs and LVD were not independent
prognostic factors.
Conclusion:
HMGB1 proteins and TAMs were highly expressed in CSCC.
Patients who exhibit increased
HMGB1 expression or increased TAM count consequently show enhanced LVD expressions, increased
lymph node metastasis, and poor
prognosis.
