ABSTRACT
AIM:
To analyze the expression profile of
long non-coding RNA (
lncRNA) in the
liver tissues of drug-induced liver injury ( DILI) and immune
liver injury ( ILI) .
METHODS:
The
technique of
lncRNA microarray was used to inspect the
lncRNA expression profile in the
mouse liver tissues that the
liver injury was induced by
acetaminophen or
concanavalin A .The raw data of
lncRNA were pretreated for normalization .
RESULTS:
Compared with normal hepatic
tissue, the
lncRNA which had more than 1.5-fold variation and significant difference (P<0.05) by
statistical analysis were regarded as
lncRNA with differential expression .A total of 68
lncRNA with differential expression were found in the hepatic
tissues of DILI, with 21 increased more than 1.5 folds and 47 reduced more than 1.5 folds.A total of 60
lncRNA with differential expression in the
liver tissues of ILI were observed , with 17 increased more than 1.5 folds and 43 reduced more than 1.5 folds.In all
lncRNA, 8 was simultaneously up-regulated in 2
liver injury models ,
accounting for 38%and 47%respectively, while 28 was simultaneously down-regulated in 2
liver injury models,
accounting for 59%and 65%re-spectively .
CONCLUSION:
lncRNA expression profiles of DILI and ILI change significantly in comparison with normal hepatic
tissue , and there are also differences between 2 hepatic damage models .The simultaneous changes of
lncRNA may participate in the same or
similar pathophysiological process , while the differences may be involved in relatively particular mechanisms .
