ABSTRACT
OBJECTIVE:
To establish
fingerprint of Zhizi jinhua pills(ZZJHW)and analyze the relationship of it with
in vitro antioxidant activity,in order to provide the basis for the
quality control of them.
METHODS:
HPLC method was adopted. The sep-aration was performed on a Sinochrom ODS-BP C18(200 mm×4.6 mm,5 μm)column with mobile phase consisted of 0.2%
acetic acid(containing 3 mmol/L
sodium heptanesulfonate
solution)-acetonitrile(gradient elution)at the
detection wavelength of 254 nm and flow rate of 0.8 ml/min. The column
temperature was controlled at 38 ℃,and
injection volume was 10 μl. The“Chromato-graphic
Fingerprint Similarity Evaluation System for TCM”(2012.130723 edition) issued by
Chinese Pharmacopoeia Commission was used to evaluate the similarity of the 12 batches of ZZJHW using baicalin as reference peak so as to attribute the common peak of
fingerprint. DPPH
free radical scavenging assay was used to investigate the
in vitro antioxidant activity of 12 batches of ZZJHW,and the relationship between its
fingerprint and
antioxidant activity was studied.
RESULTS:
The
fingerprint of 12 batches of ZZJHW was established and the similarity between the
fingerprint of ZZJHW with their reference
fingerprint were all above 0.9 (except S1,S2,S3,S12). 30 common peaks were marked,all of which were assigned to the herbs.
Antioxidant experiment result showed the differences in the
antioxidant capacity among different batches of ZZJHW;spectrum effect relationship showed that 13 common peaks were positively related with
oxidation activity and 17 common peaks negatively related with it;among known com-ponents,
oxidation activity components were mainly from
Lonicera japonica,
Scutellaria baicalensis and
Rheum palmatum. CON-CLUSIONSThe spectrum effect relationship of established
fingerprint with its
antioxidant activity can provide reference for the
quality control of ZZJHW.