ABSTRACT
Background and
purpose:
As a member of the
catenin family,
Delta-catenin protein could promote proliferation and invasion of
tumor cells, but the accurate mechanism of
Delta-catenin promoting
cell proliferation is not clear. In the present study, we illustrated that
Delta-catenin’s effect on
cell apoptosis and their relationship with
mitogen-activated protein kinase (MAPK) signaling pathway, and the possible mechanism was also explored for
Delta-catenin promoting invasion and proliferation of
tumor cells.
Methods:
The alterations of p38 and c-jun N-terminal rinasel JNK
protein activity were detected in SPC and SK
lung cancer cell lines with
Delta-catenin overexpression or not, by
Western blot method. At the same
time, the apoptotic number of
tumor cells was also examined by FCM
method. Furthermore, the number of invasive
tumor cells was examined by Matrigel invasive experiment.
Results:
Compared with untreated group and empty vector group, the activity of p38
protein was unchanged in
lung cancer cell lines with
Delta-catenin overexpressed (P>0.05), but the activity of JNK
protein was decreased signiifcantly (P<0.05),meanwhile, apoptotic proportion of
tumor cells were also reduced (P<0.05), and invasive
ability of
tumor cells was enhanced signiifcantly (P<0.05).
Conclusion:
Delta-catenin probably decreases
apoptosis number of
lung cancer cells via inhibiting the activity of
JNK pathway, and then promotes invasive
ability of
tumor cells.