The influence of CO_2 insufflation on the expression of adhesion molecules on colon cancer cell / 中华消化内镜杂志

ABSTRACT

Objective To investigate the influence of CO_2-insufflation on the expression of some adhesion molecules.Methods With an artificial pneumoperitoneum model in vitro, SW1116 colon carcinoma cells were exposed to CO_2-insufflation at different pressure (6, 9, 12 and 15 mmHg) for 1 hour. Control groups were exposed to room air. E-cadherin, ICAM-1, E-selectin, CD44 and CD44v6 were measured 0, 12, 24, 48 and 72 hours after CO_2-insufflation using flowcytometry and the mRNA level of these adhesion molecules were detected by real-time PCR.Results In transcription level the expression of ICAM-1 and CD44v6 on SW1116 cell line increased significantly in 6mmHg CO_2-insufflation group, in 9mmHg and 12mmHg group, E-cadherin,CD44 and CD44v6 increased significantly, and when the pressure was 15mmHg, CD44v6 increased (P