Effect of bone marrow stem cells mobilized by recombinan human granulocyte colony-stimulating factor on brain edema in rats after intracerebral hemorrhage / 中国组织工程研究

ABSTRACT

AIM: Research report that bone marrow stem cells mobilized by recombinan human granulocyte colony-stimulating factor (rhG-CSF) can migrate to lesion spot of infarction, thus decrease brain edema and brain injury after cerebral ischemia. But the report about the effect of drug on brain edema after cerebral hemorrhage is rare. This study investigated the effects of bone mar- row stem cells mobilized by rhG-CSF on reducing formation of brain edema and downregulation of matrix metalloproteinase (MMP)-9 in the peripheral area after intracerebral hemorrhage (ICH) in rats. METHODS:The experiments were performed at the Central Laboratory of Luzhou Medical College from March to November in 2006. ① 144 healthy male SD rats, (300?20)g, were provided by Animal Department of Luzhou Medical College. The experimental procedures of disposing animals were accorded with ethical standards. ②Experimental rats were assigned randomly into a sham operation group, a ICH group and a treatment group, equally. According to the method of Yang, rat models of ICH were made by the method cutting off tail of rat to obtain autoblood in the ICH and treatment groups. Rats in the sham operation group received saline instead of autoblood. Rats in the treatment group were administered with rhG-CSF (60 ?g/kg) by intrap- eritoneal injection after 1 hour. ③The water contents and MMP-9 were measured in each group by immunohistochemical method. RESULTS:Of 144 rats, 16 rats dropped out, among which 7 rats were estimated as 0 grade and 9 rats died, and all were supple- mented. ①The water contents were higher in the ICH group than in the sham operation group (P