ABSTRACT
Objective:
To establish the
method for the
determination of
emodin and danshensu Pinggan oral liquid.
Methods:
The contents were determined by
HPLC. The chromatographic conditions for
emodin were as follows an Agilent Eclipse XDB-C18 column (150 mm × 4. 6 mm, 5μm) was used and the mobile phase was
methanol-
water (80 ∶20). The
detection wavelength was 287 nm and the flow rate was 1. 0 ml·min-1 . The column
temperature was 25℃ and the
injection volume was 20 μl. The chromatographic condi-tions for Danshensu were as followsan Agilent Eclipse SB-C18 column (150 mm × 4. 6 mm, 5 μm) was used and the mobile phase was
methanol-1%
acetic acid solution (2 ∶98). The
detection wavelength was 280 nm and the flow was 1. 0 ml·min-1. The column
temperature was 30℃ and the
injection volume was 20 μl.
Results:
Emodin had a good linear relationship within the range of 26. 75-428. 00 μg·ml-1(r=0. 999 9). The average recovery was 102. 7% and RSD was 0. 76% (n=6).
Sodium Danshensu had a good linear relationship within the range of 10.17-162.77 μg·ml-1(r =0.999 9). The average recovery was 100.5% and RSD was 1. 47% (n=6).
Conclusion:
The
method is simple, accurate and reproducible, which can be used for the
quality control of the prep-aration.