ABSTRACT
Background:
The
incidence of
gastric cancer is gradually rising in recent years,
long non-coding RNA taurine up-regulated
gene 1 (TUG1)may have certain effects on the occurrence and progression of
gastric cancer.
Aims:
To study the expression TUG1 in
gastric cancer tissue and its effect on
prognosis of
patients with
gastric cancer,and study the correlation between TUG1 and p27,
cyclin D1.
Methods:
Surgically resected
gastric cancer tissues and corresponding distal normal
tissues of 48
gastric cancer patients from June 2013 to December 2013 at Qingdao
Municipal Hospital were collected. qRT-
PCR was used to detect the
mRNA expression of TUG1,and its relationship with clinicopathological features was analyzed.
Protein expressions of p27 and
cyclin D1 were determined by
Western blotting,and correlation with expression of TUG1 was analyzed. Kaplan-Meier was used to analyze the relationship between expression of TUG1 and
prognosis.
Results:
The
mRNA expression of TUG1 in
gastric cancer tissues was significantly higher than that in corresponding normal
tissues (6. 18 ± 0. 19 vs. 5. 09 ± 0. 16,P < 0. 05),and was not correlated with
gender,age,
tumor size,but correlated with
lymph node metastasis,
tumor differentiation and
TNM staging (P < 0. 01). The
protein expression of p27 was significantly decreased in
gastric cancer tissues than in normal
tissues (0. 1709 ± 0. 0212 vs. 0. 3087 ± 0. 0252,P < 0. 01),while
protein expression of
cyclin D1 was significantly increased (0. 3417 ± 0. 0271 vs. 0. 2417 ± 0. 0173,P < 0. 01),and the expression of p27 was negatively correlated with expression of
cyclin D1 in
gastric cancer tissues (r = - 0. 897,P < 0. 01). The expression of TUG1 was negatively correlated with expression of p27 (r = - 0. 730,P < 0. 01),and was positively correlated with expression of
cyclin D1 (r = 0. 809,P < 0. 01)in
gastric cancer tissues. The median
survival time was shorter in
gastric cancer patients with high-expressed TUG1 than in
patients with low-expressed TUG1 (P < 0. 05).
Conclusions:
Long non-coding RNA TUG1
plays a
role of
cancer gene in the development of
gastric cancer through p27 /
cyclin D1 pathway.
Detection of expression of TUG1 has important significance on the prediction of
prognosis of
gastric cancer patients.