ABSTRACT
Objective:
To analyze whether the OAZI-1 (
ornithine decarboxylase antizyme inhibitor-1)
protein complex isolated from
tumor cells could induce specific antitumor effects in the experiment
mice .
Methods:
OAZI-1
protein complexes were isolated from B16-F1
melanoma cells by immune
magnetic beads coated with OAZI-1 antibody and used as the
vaccine to immune the C 57BL/6
mice.After
immunization,the
mice were inoculated subcutaneously with live B 16-F1
cells and then
tumor formation and
growth were ob-served.
ELISA was used to determine the level of
cytokine IFN-γin the
serum of immunized
mice.
Lactate dehydrogenase assay (LDH) was performed to evaluate
killing effect of
spleen lymphocytes on B 16-F1
cells.The
mice immunized by purified OAZI-1 from prokaryotic expression and PBS were used as controls in the
animal experiment .
Results:
Compared with the control
mice ,the
spleen lymphocytes ( effector
cells ) from the
mice inoculated with OAZI-1
protein complexes had stronger
killing ability on B 16-F1
cells (target
cells).At three different effectortarget ratio (101,501,1001),the
killing ability of these
spleen lymphocytes were 46.2%, 59.5%and 92.5% respectively,which was significantly higher than the
spleen lymphocytes from the
mice inoculated with purified AZIN-1
protein (36.1%,26.8% and 45.9%) or inoculated with PBS (24.6%,24.0% and 27.2%).In addition,the content of
serum anti-
tumor cytokine IFN-γwas also significantly higher in the
mice inoculated with OAZI-1
protein complexes (538.3 pg/ml) than the
mice inoculated with purified AZIN-1 ( 256.2 pg/ml ) or with PBS ( 131.0 pg/ml ) .When B16-F1 live
cells were subcutaneously inoculated into the immunized
mice described above ,the
tumor formation rate was only 40%in the
mice immunized with OAZI-1
protein complex ,but 100%in the
mice immunized with PBS or purified OAZI-1.The
growth of inoculated
tumors in the
mice immunized with OAZI-1
protein complex was also much slower than the control
mice .
Conclusion:
The results in this study suggest that the OAZI-1
protein complex isolated from B 16-F1
tumor cells could contain some
tumor antigens .When used as
tumor vaccine to inoculate
mice ,this complex can induce anti-
tumor immune
killing activity in experimental
animals .