miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 171-185, 2018.
Article
在 En
| WPRIM
| ID: wpr-690672
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes.</p><p><b>METHODS</b>The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.</p><p><b>RESULTS</b>Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1.</p><p><b>CONCLUSION</b>Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.</p>
Key words
全文:
1
索引:
WPRIM
主要主题:
Physiology
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Esophageal Neoplasms
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Cell Communication
/
China
/
Risk
/
MicroRNAs
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PTEN Phosphohydrolase
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Exosomes
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Genetics
/
Metabolism
研究类型:
Etiology_studies
/
Risk_factors_studies
限制:
Aged
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Female
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Humans
/
Male
国家/地区名称主题:
Asia
语言:
En
期刊:
Biomedical and Environmental Sciences
年:
2018
类型:
Article