Co-culture with dental stem cells ameliorates neuronal apoptosis induced by 1-methyl-4-phenyl pyridinium / 中国组织工程研究

ABSTRACT

BACKGROUND: How to ameliorate neural injury by dental pulp stem cells is of clinical benefit for neurodegenerative diseases. OBJECTIVE: To investigate the effect of co-culture with dental pulp stem cells (DPSCs) on 1-methyl-4-phenyl pyridinium (MPP+)-induced apoptosis of neurons. METHODS: Human DPSCs were isolated and identified by morphological observation, adipogenic and osteogenic differentiation. Rat midbrain neurons were isolated and co-cultured with DPSCs by Transwell. After treatment with MPP+for 48 hours, the neurons were observed in morphology, and the effect of DPSCs on neuronal apoptosis induced by MPP+was detected by western blot and TUNEL staining. RESULTS AND CONCLUSION: DPSCs were spindle-like cells capable of differentiating into adipogenesis and osteogenesis. The apoptosis of neurons induced by MPP+were alleviated via co-culture with DPSCs. Further detection by western blot showed that the neurons treated with MPP+reduced the expression of Bcl-2 and promoted the expression of Bax and cleaved caspase3; however, the changes in the expression of these proteins were reduced under the co-culture system of DPSCs. All the above results reveal that DPSCs may repair or prevent neuronal injury induced by MPP+through paracrine actions.