Cholera toxin mediated regulation of the expression of Gq alpha and G11 alpha GTP binding proteins
Experimental & Molecular Medicine
; : 89-94, 1999.
Article
在 En
| WPRIM
| ID: wpr-70471
Responsible library:
WPRO
ABSTRACT
Previously it has been shown that persistent activation of the stimulatory adenylyl cyclase pathway with cholera toxin (CT) downregulates the Gs alpha polypeptide (80%) in a cAMP-independent manner in C6 glioma cells (Shah, 1997). This study was conducted to examine the short and long term effects of CT on the regulation of pertussis toxin-sensitive and -insensitive G proteins and their transcripts in C6 glioma cells. Treatment of C6 cells with CT (100 ng/ml) up to 16 h had no effect on either Gi or Gq/11 alpha proteins. However, prolonged exposure (24-48 h) caused increased expression of Gi (20-30%) and Gq/11 alpha proteins (40%). Urea gradient gels, which can separate Gq alpha and G11 alpha proteins, revealed that prolonged CT treatment increased the expression of both of these G proteins. The CT-mediated enhanced expression of Gq alpha and G11 alpha proteins was accompanied by increased mRNA levels of these proteins as determined by RT/PCR. Cyclic-AMP elevating agents like forskolin (10 microM) and db-cAMP (1 mM) mimicked the effect of CT on Gi but not Gq/11 alpha proteins. These studies show long term cAMP-dependent regulation of Gi and cAMP-independent expression of Gq/11 alpha proteins in C6 glioma cells.
Key words
全文:
1
索引:
WPRIM
主要主题:
Colforsin
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RNA, Messenger
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Gene Expression Regulation
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Cholera Toxin
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Blotting, Western
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Cyclic AMP-Dependent Protein Kinases
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Bucladesine
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GTP-Binding Proteins
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Reverse Transcriptase Polymerase Chain Reaction
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Glioma
限制:
Animals
语言:
En
期刊:
Experimental & Molecular Medicine
年:
1999
类型:
Article