Biological and Genetic Characteristics of Clinically Isolated Enterobacter cloacae with Multidrug Resistance
Laboratory Medicine Online
; : 99-106, 2018.
Article
在 En
| WPRIM
| ID: wpr-715910
Responsible library:
WPRO
ABSTRACT
BACKGROUND: From January 2014 to December 2015, 69 clones of Enterobacter cloacae showing multidrug resistance to six classes of antimicrobial agents were collected from two medical centers in Korea. METHODS: Minimum inhibitory concentrations were determined using the E-test method, and 17 genes were detected using polymerase chain reaction (PCR). The epidemiological relatedness of the strains was identified using repetitive element sequence-based PCR and multilocus sequence typing. RESULTS: The 69 E. cloacae clones produced extended spectrum β lactamase (ESBL) and AmpC and showed multidrug resistance to cefotaxime, ceftazidime, and aztreonam. We identified the following sequence types: ST56 of type VI for ESBL SHV (N=12, 17.4%); ST53, ST114, ST113, and ST550 of types I, IV, VI, and VII, respectively, for CTX-M (N=11, 15.9%); and ST668 of type III for the carbapenemase NDM gene (N=1, 1.5%). The AmpC DHA gene (N=2, 2.89%) was confirmed as ST134, although its type was not identified, whereas EBC (MIR/ACT; N=18, 26.1%) was identified as ST53, ST24, ST41, ST114, ST442, ST446, ST484, and ST550 of types V, I, III, IV, VII, and VI, respectively. The formed subclasses were bla CTX-M-3 and bla CTX-M-22 by CTX-M-1, bla CTX-M-9 and bla CTX-M-125 by CTX-M-9, bla DHA-1 by DHA, and bla MIR-7 and bla ACT-15,17,18,25,27,28 by EBC (MIR/ACT). CONCLUSIONS: There were no epidemiological relationships between the gene products and the occurrence of resistance among the strains.
Key words
全文:
1
索引:
WPRIM
主要主题:
Aztreonam
/
Microbial Sensitivity Tests
/
Cefotaxime
/
Ceftazidime
/
Polymerase Chain Reaction
/
Cloaca
/
Enterobacter cloacae
/
Clone Cells
/
Drug Resistance, Multiple
/
Enterobacter
研究类型:
Prognostic_studies
国家/地区名称主题:
Asia
语言:
En
期刊:
Laboratory Medicine Online
年:
2018
类型:
Article