Role of Regulators of G-Protein Signaling 4 in Ca2+ Signaling in Mouse Pancreatic Acinar Cells
The Korean Journal of Physiology and Pharmacology
; : 383-388, 2011.
Article
在 En
| WPRIM
| ID: wpr-728316
Responsible library:
WPRO
ABSTRACT
Regulators of G-protein signaling (RGS) proteins are regulators of Ca2+ signaling that accelerate the GTPase activity of the G-protein alpha-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca2+ oscillations. However, the role of RGS4 in Ca2+ signaling in pancreatic acinar cells is unknown. In this study, we investigated the mechanism of GPCR-induced Ca2+ signaling in pancreatic acinar cells derived from RGS4-/- mice. RGS4-/- acinar cells showed an enhanced stimulus intensity response to a muscarinic receptor agonist in pancreatic acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca2+ oscillations. RGS4-/- cells also showed increased expression of sarco/endoplasmic reticulum Ca2+ ATPase type 2. However, there were no significant alterations, such as Ca2+ signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4-/- mice. These results indicate that RGS4 protein regulates Ca2+ signaling in mouse pancreatic acinar cells.
Key words
全文:
1
索引:
WPRIM
主要主题:
Pancreas
/
Reticulum
/
Proteins
/
Receptors, Muscarinic
/
Calcium-Transporting ATPases
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GTP-Binding Proteins
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RGS Proteins
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Acinar Cells
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GTP Phosphohydrolases
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Amylases
限制:
Animals
语言:
En
期刊:
The Korean Journal of Physiology and Pharmacology
年:
2011
类型:
Article