Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells
The Korean Journal of Physiology and Pharmacology
; : 307-311, 2014.
Article
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| ID: wpr-728464
Responsible library:
WPRO
ABSTRACT
In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 microM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
Key words
全文:
1
索引:
WPRIM
主要主题:
Phosphorylation
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Scopoletin
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Vitiligo
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Cell-Free System
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Blotting, Western
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Monophenol Monooxygenase
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Cyclic AMP Response Element-Binding Protein
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Cirsium
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Microphthalmia-Associated Transcription Factor
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Melanins
语言:
En
期刊:
The Korean Journal of Physiology and Pharmacology
年:
2014
类型:
Article