ABSTRACT
@#
AIM:
To observe the expression of
erythropoietin(EPO)on the corneal of
rabbit and evaluate the inhibition effect of EPO
siRNA on
corneal neovascularization(CNV). <p>
METHODS:
Totally 22 healthy
rabbits were randomly divided into 2 groups, which were experimental group and normal
control group. Both
eyes of
rabbits in experimental group were chosen to establish
corneal neovascularization model by
alkali burn. The morphologic change of corneal was observed with
slit lamp microscope and the area of CNV was calculated every day. After
alkali burn, the right
eye of the experimental group was accepted EPO
siRNA injection under the
conjunctiva, and the left
eye was assigned to be experimental
control group. The corneal with CNV was collected for
immunohistochemistry at 3d, 7d, 14d, 21d after
alkali burn, and the expression of EPO was measured. <p>
RESULTS:
CNV began growing at the 3d after
alkali burn in experimental group, and it was vigorous growing at 7d-14d period. The result of
immunohistochemistry shows that the expression of EPO increased after the operation. Compared with experimental group, the
rabbits who were treated by EPO
siRNA was found with less neovascularization on their corneal, and the expression of EPO decreased. There were statistical significance between the two group at different
time(<i>P</i><0.05). <p>
CONCLUSION:
EPO is likely to
play an important
role on CNV
growth, and EPO
siRNA can inhibit the
growth of CNV by restraining the expression of EPO.
