Pulegone Exhibits Anti-inflammatory Activities through the Regulation of NF-κB and Nrf-2 Signaling Pathways in LPS-stimulated RAW 264.7 cells
Natural Product Sciences
; : 28-35, 2018.
Article
在 En
| WPRIM
| ID: wpr-741600
Responsible library:
WPRO
ABSTRACT
Pulegone is a naturally occurring organic compound obtained from essential oils from a variety of plants. The aim of this study was to investigate the anti-inflammatory effects through the inhibitory mechanism of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK) pathways and the activation of nuclear factor erythroid 2-related factor 2 (Nrf2)/ heme oxygenase (HO)-1 pathways in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Results revealed that pulegone significantly inhibited NO production as well as iNOS and COX-2 expressions. Meanwhile, western blot analysis showed that pulegone down-regulated LPS-induced NF-κB and MAPKs activation in RAW 264.7 cells. Furthermore, the selected compound suppressed LPS-induced intracellular ROS production in RAW 264.7 cells, while the expression of stress response gene, HO-1, and its transcriptional activator, Nrf-2 was upregulated upon pulegone treatment. Taking together, these findings provided that pulegone inhibited the LPS-induced expression of inflammatory mediators via the down-regulation iNOS, COX-2, NF-κB, and MAPKs signaling pathways as well as up-regulation of Nrf-2/HO-1 indicating that pulegone has a potential therapeutic and preventive application in various inflammatory diseases.
Key words
全文:
1
索引:
WPRIM
主要主题:
Oils, Volatile
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Down-Regulation
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Up-Regulation
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Blotting, Western
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NF-kappa B
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Prostaglandin-Endoperoxide Synthases
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Mitogen-Activated Protein Kinases
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Nitric Oxide Synthase Type II
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Heme Oxygenase (Decyclizing)
语言:
En
期刊:
Natural Product Sciences
年:
2018
类型:
Article