Anti-scarring effect of rapamycin in rabbits following glaucoma filtering surgery / 南方医科大学学报
Journal of Southern Medical University
; (12): 1389-1394, 2018.
Article
在 Zh
| WPRIM
| ID: wpr-771462
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To study the anti- scarring effect of rapamycin in rabbits receiving glaucoma filtering surgery.@*METHODS@#Ninety-six Chinchilla rabbits were randomized equally into 3 rapamycin treatment groups and one control group. All the rabbits underwent trabeculectomy, after which the rabbits in the 3 rapamycin groups were treated with eye drops containing 1%, 3%, or 5% rapamycin in the operated eyes, and those in the control groups were given castor oil 4 times a day. The intraocular pressure (IOP) and inflammatory reaction in the treated eyes were observed, and the PCNA-positive cells in the filtering bleb were detected using immunohistochemistry. RTFs isolated from the Tenon's capsule of the rabbits were cultured , and the expressions of caspase-3, caspase-8, and caspase-9 in the fibroblasts were detected after treatment with different concentrations of rapamycin.@*RESULTS@#The IOP was significantly lower in rapamycin-treated group than in the control group after the surgery ( < 0.05). The counts of the PCNA-positive cells were significantly lower in rapamycin-treated rabbits than in the control group ( < 0.05). Rapamycin treatment dose-dependently increased the expressions of caspase-3 and caspase- 9 at both the mRNA ( < 0.001) and protein ( < 0.001) levels without causing significant changes in the expressions of caspase-8.@*CONCLUSIONS@#Rapamycin can inhibit excessive proliferation of the fibroblasts in the filtering bleb to reduce scar formation after glaucoma filtration surgery in rabbits. Rapamycin also increases the expressions of caspase-3 and caspase-9 to induce apoptosis of the RTFs.
Key words
全文:
1
索引:
WPRIM
主要主题:
Postoperative Complications
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General Surgery
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Trabeculectomy
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Random Allocation
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Glaucoma
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Cicatrix
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Proliferating Cell Nuclear Antigen
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Filtering Surgery
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Sirolimus
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Therapeutic Uses
限制:
Animals
语言:
Zh
期刊:
Journal of Southern Medical University
年:
2018
类型:
Article