Protective effects of exogenous vitamin D on nerve injury in mice with cerebral ischemia/reperfusion / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 300-303, 2019.
Article
在 Zh
| WPRIM
| ID: wpr-776510
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effects of 1,25-dihydroxyvitamin D3 (1,25-VitD3) supplementation on cerebral injury after ischemia/reperfusion (I/R) in mice with middle cerebral artery occlusion (MCAO).@*METHODS@#Male C57BL6 mice were randomly divided into Sham group, Vehicle group and 1,25-VitD3 group, with 10 mice in each group. Vehicle group and 1,25-VitD3 group were given MCAO for 1 hour, and then killed after reperfusion for 24 hours. Mice in 1,25-VitD3 group were treated with 1,25-VitD3 at the dose of 100 ng/(kg·d) by injected intraperitoneally for 5 days before MCAO operation. Cerebral ischemic penumbra areas of each group were collected for TTC staining, RT-PCR, TTC staining and immunohistochemistry assay. The function defect of mice was evaluated by using neurological function score.@*RESULTS@#Compared with the sham group, the volume of cerebral infarction in Vehicle group was increased significantly, and the expressions of IL-6, IL-1beta and Gp91phox in brain tissues were increased significantly (P<0.05); compared with Vehicle group, supplementation of 1,25-VitD3 reduced the volume of cerebral infarction by about 50% in I/R mice (P<0.05), and the expressions of IL-6, IL-1beta and Gp91phox in brain tissues of 1,25-VitD3 group were decreased significantly (P<0.05). The expression of Foxp3, a T-regulatory cell marker, was significantly increased in the brain of mice (P<0.05), while the expression of Rorc, a transcription factor, was significantly decreased (P<0.05), suggesting that Th17/gamma Delta T-cell response was reduced and the number of neutrophils in the brain injury site of mice was significantly reduced (P<0.05).@*CONCLUSION@#Vitamin D could alleviate the development of cerebral infarction after arterial occlusion (MCAO) reperfusion, and its mechanism may be through regulating the inflammatory response in mouse brain I/R.
全文:
1
索引:
WPRIM
主要主题:
Pharmacology
/
Vitamin D
/
Brain
/
T-Lymphocytes
/
Reperfusion Injury
/
Random Allocation
/
Cytokines
/
Rats, Sprague-Dawley
/
Protective Agents
/
Infarction, Middle Cerebral Artery
限制:
Animals
语言:
Zh
期刊:
Chinese Journal of Applied Physiology
年:
2019
类型:
Article