ABSTRACT
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Objective:
To explore the expression of IQGAP1 (
Ras GTPase-activating-like
protein containing IQ domain) in
esophageal squamous cell carcinoma (ESCC)
tissues and
cell lines and its effect on the proliferation and invasion of TE-2
cell.
Methods:
Totally 125 pairs of
cancer tissues and para-cancerous
tissues from ESCC
patients,
who underwent surgical resection inAffiliated
Tumor Hospital of Zhengzhou
University from January 2015 to December 2016, were included in this study; in addition, ESCC
cell lines (TE-2, TE3, ECA109) and normal esophageal
epithelial cell line Het-1A were also collected. The expression of IQGAP1 was detected by immunohistochemical
staining and its relationship with cliniopathological features was also analyzed. IQGAP1
mRNA and
protein expressions in ESCC
cell lines were detected by Real-
time quantitative
PCR (qPCR) and
Western blotting, respectively. TE-2
cells were transfected with si-IQGAP1 (positive
transfection group) and si-CTRL (negative
control group)
plasmids, and the effects of IQGAP1 silencing on the proliferation and invasion of TE-2
cells were detected by MTT and Transwell assay. The expressions of
E-cadherin and Ncadherin were detected by
Western blotting.
Results:
The positive expression rate of IQGAP1 in ESCC
tissues was significantly higher than that in para-cancerous
tissues (P<0.05), which was closely related to
tumor stage and histologic grade (all P<0.05). The mRNAand
protein expressions of IQGAP1 in TE-2, TE-3 and ECA109
cells were significantly higher than those in Het-1Acells (all P<0.05).After IQGAP1 was silenced, compared with the negative
control group and the blank group, the expression of IQGAP1 mRNAand
protein in the positive
transfection group significantly decreased (all P<0.05); the proliferation and invasiveness of TE-2
cells significantly decreased (all P<0.05); E-cardherin was up-regulated while N-cardherin was down-regulated (all P<0.05) in the positive interference group.
Conclusion:
IQGAP1 is highly expressed in ESCC
tissues, and si-IQGAP1 can inhibit the proliferation and invasion of TE-2
cells, which
plays an important
role in the occurrence and development of ESCC.
