ABSTRACT
Objective:
To isolate and identify the chemical constituents from 95%
ethanol extracts from stems of
Zanthoxylum bungeanum.
Method:
The stems (25 kg) were extracted with 95%
ethanol under reflux for three times and then filtrated,and the combined filtrates were concentrated under
vacuum to get the extracts. After
suspension with
water,the extracts were extracted with
petroleum ether,
dichloromethane,ethyl
acetate and
n-butanol successively,and five corresponding parts were obtained. The parts of
petroleum ether,
dichloromethane,ethyl
acetate were
separated in a
similar way to get pure compounds. By the
method the subjects were first chromatographed on
silica gel column respectively,then the selected sub-fractions were further
separated by Sephadex LH-20, and finally purified on preparation
HPLC to get the monomer compound. The
n-butanol part was first treated with macroporous resin D101,and then the sub-fractions were further purified by almost the same
method as mentioned above. The structures of these compounds were determined by spectrum
technology as IR,MS,
1H-NMR,13 C-NMR.
Result:
Ten compounds were isolated from Z. bungeanum stems and identified as follows(+)-magnoflorine(1),(-)-marmesin(2),(-)-columbianetin(3),(-)-decursinol(4),lupeol(5),α-amyrin(6),β-amyrin(7),δ-amyrin(8),
quercetin(9),
rutin(10). Among them, 1 was
alkaloid, 2,3,4 were coumarin, 5,6,7,8 were
triterpenoid, 9,10 were
flavonoids.
Conclusion:
Compounds 1-8 were isolated from this
plant for the first
time.