Toxic effects of strychnine and strychnine N-oxide on zebrafish embryos / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 760-767, 2014.
Article
在 En
| WPRIM
| ID: wpr-812203
Responsible library:
WPRO
ABSTRACT
AIM@#The application of strychnine (S) is limited due to its toxicity; strychnine N-oxide (SNO) is a derivative of strychnine. The aim was to employ zebrafish embryos to investigate and compare the developmental toxicity induced by S and SNO.@*METHODS@#The toxicity of S and SNO was examined through the hatching rate and survival rate. Morphological changes of the zebrafish were observed with a dissecting microscope. Apoptosis was detected through acridine orange (AO) staining and flow cytometry. Apoptotic genes were measured by RT-PCR.@*RESULTS@#Embryo malformation was observed in the embryos exposed to S at 200 μmol·L(-1). When SNO concentration was increased to 1 mmol·L(-1), scoliolosis, and pericardial edema could be seen in some embryos. Results from fluorescence microscopy and flow cytometry analysis showed that S at 200 μmol·L(-1) induced apoptosis, whereas the apoptotic rate in the SNO-treated group (200 μmol·L(-1)) was much lower than that in the S group. RT-PCR analysis showed that p53 mRNA expression and the ratio of Bax/Bcl-2 in the S group were significantly altered compared with the control group (*P < 0.05). Moreover, Bax mRNA expression in both S and SNO group were significantly different from that in the control group (**P < 0.01).@*CONCLUSION@#These results lead to the conclusion that SNO has significantly lower toxicity than S in zebrafish embryos.
Key words
全文:
1
索引:
WPRIM
主要主题:
Strychnine
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Zebrafish
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Drugs, Chinese Herbal
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Chemistry
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Embryology
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Tumor Suppressor Protein p53
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Apoptosis
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Oxidative Stress
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Proto-Oncogene Proteins c-bcl-2
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Cyclic N-Oxides
限制:
Animals
语言:
En
期刊:
Chinese Journal of Natural Medicines (English Ed.)
年:
2014
类型:
Article