Characteristics and expression of Mip5, a novel gene associated with myocardial ischemia/reperfusion in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 515-520, 2005.
Article
在 Zh
| WPRIM
| ID: wpr-813516
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To determine the characteristics of a novel gene Mip5 (GenBank accession number AY553870) and its expression under physiological and pathological conditions.@*METHODS@#The characteristics of Mip5 were analyzed by bioinformatic programs including BLAST, spidey, psort, ClustalW and so on. RT-PCR was performed to detect Mip5 expression.@*RESULTS@#Bioinformatic analysis showed that Mip5 gene lied in the 13th chromosome and contained 8 exons and 7 introns, its open reading frame contained 909 bp and its protein production was 302 amino acid residues including 6 kelth domains. Under normal conditions, MIP5 expressed abundantly in the heart, brain and kidney, but its expression could not be detected in the liver and muscle. Expression of Mip5 gene was increased significantly after ischemia-reperfusion compared with the sham groups, and reached its peak at 3 h and recovered at 12 h after the reperfusion. Conclusion Mip5 gene is a novel gene containing a putative open reading frame of 302 amino acids residues and may play an important role in rat cardiomyocytes suffering ischemia processing.
全文:
1
索引:
WPRIM
主要主题:
Chromosomes, Human, Pair 13
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Molecular Sequence Data
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Base Sequence
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Myocardial Reperfusion Injury
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Open Reading Frames
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Amino Acid Sequence
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Myocardial Ischemia
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DNA, Complementary
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Genetics
限制:
Animals
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Humans
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Male
语言:
Zh
期刊:
Journal of Central South University(Medical Sciences)
年:
2005
类型:
Article