Expression of Class I integrase gene in Acinetobacter baumannii and drug-resistance / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 952-957, 2008.
Article
在 Zh
| WPRIM
| ID: wpr-814169
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the expression level of class I integrase (intI 1) mRNA in Acinetobacter baumannii from biofilm cells and planktonic cultured cells ,and to analyze the drug-resistance of Class I integron positive strains.@*METHODS@#Acinetobacter baumannii were collected from hospitals,and Class I integron strains were screened by gene amplification. Total RNA of Class I integron positive strains was extracted, and the intI1 mRNA expression in the bioflim cells and planktonic cultured cells was measured by RT-PCR. Susceptibilities to antibiotics of Class I integron positive strains were also examined.@*RESULTS@#The intI1 gene mRNA was expressed under 2 conditions, and the mRNA expressed in the biofilm cells was about 4 times higher than that in the planktonic cultured cells. Among the 64 strains of Acinetobacter baumannii, 46 strains were Class I integron positive strains. The antibiotic resistance of intI1 gene cassette-positive strains was higher than that of gene cassette-negative strains.@*CONCLUSION@#The intI1 gene mRNA can be up-regulated in Acinetobacter baumannii biofilm cells.Class I integron plays an important role in drug resistance. It is much easier to capture gene cassettes for bacteria under biofilm condition.
全文:
1
索引:
WPRIM
主要主题:
Acinetobacter Infections
/
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
Drug Resistance, Multiple
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Biofilms
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Integrases
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Acinetobacter baumannii
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Genetics
/
Microbiology
限制:
Humans
语言:
Zh
期刊:
Journal of Central South University(Medical Sciences)
年:
2008
类型:
Article