miR-181a promotes the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 789-795, 2016.
Article
在 Zh
| WPRIM
| ID: wpr-814962
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the role of miR-181a in promoting the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A.
@*METHODS@#The level of miR-181a in 30 human osteosarcoma tissues and corresponding bone tissues was detected by real-time PCR, and the correlation between the level of miR-181a and clinicopathological characteristics of osteosarcoma was analyzed. Osteosarcoma cells MG-63 were transfected with chemically-synthesized miR-181a mimics and inhibitors, and the proliferation, migration and invasion of MG-63 cells were detected by MTT and Transwell assay. The specific binding ability of miR-181a to RASSF1A 3'-UTR was theoretically predicted and detected by the dual luciferase reporter gene assay.
@*RESULTS@#The level of miR-181a in osteosarcoma tissues was statistically higher than that in the corresponding bone tissues (P0.05). MTT and Transwell assays showed that the growth rate, migration and invasion ability of MG-63 cells with up-regulation of miR-181a was significantly increased compared with negative control (P<0.05), while the growth rate, migration and invasion ability of MG-63 with down-regulated miR-181a was significantly decreased compared with negative control (P<0.05). Luciferase reporter gene assay showed that miR-181a targeted the 3'-UTR of RASSF1A and regulated the expression of RASSF1A.
@*CONCLUSION@#MiR-181a promotes the proliferation and metastasis of osteosarcoma cells through specifically binding to RASSF1A 3'-UTR and subsequent down-regulation of RASSF1A.
全文:
1
索引:
WPRIM
主要主题:
Transfection
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Osteosarcoma
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Down-Regulation
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Up-Regulation
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Tumor Suppressor Proteins
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MicroRNAs
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Cell Line, Tumor
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Cell Proliferation
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Real-Time Polymerase Chain Reaction
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Neoplasm Metastasis
研究类型:
Prognostic_studies
限制:
Humans
语言:
Zh
期刊:
Journal of Central South University(Medical Sciences)
年:
2016
类型:
Article