Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 620-622, 2020.
Article
在 En
| WPRIM
| ID: wpr-828972
Responsible library:
WPRO
ABSTRACT
Real-time quaking-induced conversion (RT-QuIC) assay is a newly established PrP -detecting method. The development of RT-QuIC improves the diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD), showing good sensitivity and specificity in many countries when the method was used in cerebrospinal fluid (CSF) samples. However, in China, the sensitivity and specificity of RT-QuIC has yet to be determined due to the lack of definitive diagnosis samples. Recently, 30 definitive sCJD and 30 non-CJD diagnoses were evaluated by RT-QuIC assay. In the 30 sCJD CSF samples, 29 showed positive results. By contrast, all the non-CJD samples were negative. The sensitivity and specificity of our RT-QuIC assay were 96.67% and 100%, respectively, and are comparable to other published data. Results can provide a fundamental basis for the usage of RT-QuIC assay in CJD surveillance in China.
全文:
1
索引:
WPRIM
主要主题:
Cerebrospinal Fluid
/
China
/
Creutzfeldt-Jakob Syndrome
/
Sensitivity and Specificity
/
PrPSc Proteins
/
Diagnosis
/
Diagnostic Tests, Routine
/
Methods
研究类型:
Diagnostic_studies
/
Screening_studies
限制:
Humans
国家/地区名称主题:
Asia
语言:
En
期刊:
Biomedical and Environmental Sciences
年:
2020
类型:
Article