ABSTRACT
Objective:
To investigate the expression and
clinical significance of
protein regulator of
cytokinesis 1 (PRC1) in
soft tissue sarcoma (STS) and the effects of down-regulating the expression of PRC1 on the proliferation and
cell cycle of
human liposarcoma cell by
data mining.
Methods:
Oncomine database was used to analyze the expression of PRC1 in STS
tissue and normal
tissue, which was then verified by TCGA database. The
prognosis data downloaded from OncoLnc database were used to analyze the correlation between PRC1 expression and
prognosis of STS
patients. Meanwhile, to collect PRC1 expression in STS
cells, we used publicly available data from the
Cancer Cell Line Encyclopedia (CCLE)
projects. String database was used to determine the co-expression molecules with PRC1 and map the
gene co-expression network. The expressions of PRC1 in
liposarcoma cell line SW872 and
human subcutaneous preadipocytes (HPA-s) were detected by
Western blotting and
Real-time PCR. PRC1 was silenced in SW872
cell (SW872-siPRC1) by PRC1 target
siRNA with SW872-NC
cell as its control. MTT assay was used to detect the proliferation of SW872-siPRC1 and SW872-NC
cells;
flow cytometry was used to detect the
cell cycle.
Results:
In the Oncomine database, 11 studies involved PRC1 expression in STS
tissues and normal
tissues. Compared with that of the control, the expression of PRC1 in STS
tissues was significantly higher (P<0.05). The results were consistent with those in the TCGA database. The
analysis using Oncomine database showed that the high expression level of PRC1 was associated with shorter overall
survival (P<0.05). The
analysis using CCLE database showed high expression of PRC1 in STS
cells (P<0.05). The co-expression network of PRC1 was established by String database, including 11 nodes and 55 connections. PRC1 was over-expressed in
liposarcoma cell line SW872.
Cell proliferation curve showed that compared with that of SW872-NC
cells in the
control group, the proliferation of SW872-siPRC1
cells decreased significantly after 48 h and 72 h
culture (P<0.05). Compared with SW872-NC
cell in the
control group, the G1
cell proportion of SW872-siPRC1
cells was (40.27±7.42)%, significantly lower than that of SW872-NC
cells (62.01±4.89)%. The G2/M cell proportion of SW872-siPRC1
cells was (25.65±1.54)%, which was significantly higher than that of SW872-NC
cells (8.17±0.96)% (both P<0.05).
Conclusion:
Tumor gene database
mining shows that PRC1 is highly expressed in STS
tissues and STS
cells, which is associated with the
patient's
prognosis. Silencing PRC1
gene can inhibit the proliferation of
liposarcoma SW872
cells and keep the
cells staying in G2/
M phase. PRC1
plays a
role in promoting
liposarcoma, which may provide a potential target for the clinical
treatment and
prognosis of
soft tissue sarcoma, especially
liposarcoma.