Screening and identification of immunodominant epitope of human serum albumin / 国际药学研究杂志

ABSTRACT

Objective To select the immunodominant epitope of human serum albumin (HSA) and provide the basis for setting up a special and rapid detecting method of HSA. Methods Bioinformatic method was used to compare protein sequences of human, pig, horse, ox, and ovine, and the immunodominant epitopes of HSA were predicted. The E.coli preferred codons were used todesign the DNA sequences of the selected epitopes. The genes of the epitopes were expressed after they were inserted into the PGEX-4T-2 vector. The recombinant antigens were identified and valued by HSA antibody with indirect ELISA. Results The length of the selected epitopes was H1 [126-162 amino acid (aa)], H2 (314-355aa), and H3 (373-424aa) and the relative molecular weight was 3.01×104, 3.06×104 and 3.17×104, respectively. The epitope of 373-424 aa were more active and its cross-reactivity IC50 of enzymelabeled antibody was 1.635 mg/L, which was higher than HSA (P0.05). Conlusion The immunodominant epitope of HSA is obtained, which is significant for developing a rapid and special reagent of HSA.