ABSTRACT
OBJECTIVE:
Schwann cells can promote the
regeneration of damaged
peripheral nerves and serve as
seed cells in the
engineering repair of
peripheral nerve tissue. The effective
culture and purification of
Schwann cells are the basis of clinical
treatment for
peripheral nerve injury. This
paper summarized the
literature of
culture of
Schwann cells in vitro in recent ten years and made a descriptive
review on the
research progress of
Schwann cell culture and purification, in order to provide references for
culture of
Schwann cells in vitro.
METHODS:
Literature related to
Schwann cell isolation,
culture and purification was retrieved from
PubMed, Web of
Science,
Medline databases, CNKI, Wanfang, VIP and other databases. The
keywords included “
Schwann cells; isolation;
culture; purification”. All the
papers obtained from the search were read, analyzed and judged, and finally 62
papers meeting the
standards were included.
RESULTS:
The classical
methods of
Schwann cell culture include
tissue block
culture and
enzyme digestion. Purification
methods include
mitosis resistance, immune selection, specific adhesion, pre degeneration,
cold jet, differential adherent,
laminin package, low
serum, stimulating factor,
fluorescence activated cell sorting or
magnetic activated
cell sorting, immunopanning, and extracorporeal
shock wave treatment.
CONCLUSION:
Schwann cells can be cultured and purified in various ways
in vitro, and each
method has its advantages and disadvantages. How to obtain high-purity
Schwann cells quickly and efficiently is still a challenge. Multiple
methods can be combined for purification and affective factors can be controlled for
Schwann cell proliferation as much as possible.