ABSTRACT
BACKGROUND:
Defective
dentition is a common oral
disease, if it is not treated in
time, there
will be
adverse effects such as tilting of the adjacent
teeth and elongation of the
jaws, causing occlusal disorder and interference, which
will seriously
affect the later repair. Especially in the diabetic
patients with
dentition loss, the impacts of diabetes on the occlusal elongation of the
jaw teeth, and how
osteonectin changes in this process, are still unclear.
OBJECTIVE:
To investigate the effect of diabetes on
tooth occlusion and elongation in
mice by establishing a model of the occlusion of the
jaws in diabetic
mice.
METHODS:
A diabetic model was established by
intraperitoneal injection of
streptozotocin in C57 BL/6J
mice (purchased from the
Animal Experimental Center of Shanxi Medical
University). The
mice were intraperitoneally injected with
sodium citrate buffer. Thirty
mice with successful modeling and control
mice were removed, and the three right maxillary
molars were removed to establish an
experimental model of the extensional
movement of the maxillary
teeth. After 0, 3, 6, 9 and 12 days, the right
jaw was taken. The
bone mineral density was measured by micro-CT. The number of
osteoclasts was counted by
tartrate resistant acid phosphatase staining. The expression level of
osteonectin was detected by RT-qPCR. The study was approved by the Ethical Committee of Shanxi Medical
University. RESULTS AND
CONCLUSION:
(1) With the
time increasing, the
bone mineral density of the right
mandible in the two groups was gradually increased. The
bone mineral density in the diabetic group was significantly lower than that in the
control group at 3, 6, 9 and 12 days after
surgery (P < 0.05). (2) With the
time increasing, the number of
osteoclasts in the right
mandible of both groups was gradually increased. The number of
osteoclasts in the diabetic group was significantly lower than that in the
control group at 3, 6, 9 and 12 days after
surgery (P < 0.05). (3) The expression level of
osteonectin mRNA in the right
mandible of both groups was gradually increased. The expression level of
osteonectin mRNA in the diabetic group was significantly higher than that in the
control group at 0, 3,6, 9 and 12 days after
surgery (P< 0.05). (4) These results indicate that diabetes can reduce the
bone construction ability during the extensional
movement and promote
osteonectin mRNA expression.