ABSTRACT
Objective:
To investigate the effect and the possible mechanism of Rhizoma Paridis total
saponin (RPTS) on
human gastric cancer cell line MKN-45 proliferation, migration and invasion
in vitro.
Methods:
MKN-45
cells were cultured
in vitro and treated respectively with indicated concentrations of RPTS (2.5, 5.0, 10.0, 20.0, and 40.0 μg/mL) for 24 h, and
cell viability of
cell proliferation was detected by MTT assay; The invasive and metastatic
ability of MKN-45 treated with indicated concentrations of RPTS (2.5, 5.0, 10.0 μg/mL) was detected by Transwell migration assay and
wound healing assay;
Elisa assay was employed to detect the concentrations of
MMP-9 induced by LiCl after RPTS
administration (10, 20, and 40 μg/mL) in the
cell supernatant;
Western blotting and qRT-
PCR were respectively performed to investigate the invasion and migration related
protein and
mRNA level of
VEGF, COX-2, and
GSK-3β in RPTS-treated MKN-45 after LiCl stimulation for 24 h.
Results:
Compared with the
control group, RPTS (10, 20, and 40 μg/mL) significantly inhibited the proliferation of MKN-45
cells (P < 0.05 and P < 0.001); RPTS (2.5, 5.0, 10.0 μg/mL) suppressed the invasion and migration of MKN-45
cells (P < 0.05 and P < 0.001); Compared with the model group, RPTS significantly downregulated the expression of
MMP-9 in the
cell supernatant of MKN-45
cells induced by LiCl (P < 0.05 and P < 0.01), and RPTS also decreased the
protein and
mRNA expression level of
VEGF and COX-2, but it significantly upregulated the expression of
GSK-3β at the
protein and
mRNA level (P < 0.05 and P < 0.001).
Conclusion:
RPTS
play a pivotal
role in suppressing the invasion and migration of MKN-45
cells in vitro, and its mechanism may be related to the regulating effects of the Wnt/β-
catenin pathway in the
human gastric
adenocarcinoma cell.