Fecal metabolome and gut microbiome alterations in a mouse model of senescence accelerated mouse prone 8 (SAMP8) / 中草药

ABSTRACT

Objective To investigate the alterations of fecal metabolites and intestinal flora during the aging in a mouse model of senescence accelerated mouse prone 8 (SAMP8). Methods The 1H-NMR metabonomics and metagenomics were applied to investigate the aging-related metabolic markers and intestinal flora, and Pearson correlation analysis was performed between metabolites and gut flora. Results Thirty-one endogenous metabolites were identified in the faeces of SAMP8 mice, of which 13 metabolites changed significantly compared with SAMR1 mice. Differential metabolites were mainly enriched in four metabolic pathways phenylalanine, tyrosine and tryptophan biosynthesis; valine, leucine and isoleucine biosynthesis; phenylalanine metabolism; histidine metabolism. The results showed that the diversity of intestinal flora was significantly changed and the relative abundances of 10 kinds of intestinal flora were significantly changed in 10-month-old SAMP8 mice. Correlation analysis showed that Christensenellaceae was positively correlated with phenylalanine, histidine, valine, isoleucine, and uridylic acid; Dehalobacterium was negatively correlated with tyrosine, and Planococcaceae was negatively correlated with valine. Conclusion This paper reveals the changes of fecal metabolites and gut flora in SAMP8 mice, which provides experimental evidence for the study of aging progress and anti-aging actions of drugs.