ABSTRACT
Objective:
To study the dynamic variation of Mn, Cu, Zn, Fe, K, Ca, and Mg in velamen and
stem bark of
Periploca sepium and their correlation with the
elements such as periplocin and 4-methoxysalicylic
aldehyde in different
harvesting periods, and to reveal dominant factors on the accumulation of two active components.
Methods:
The contents of periplocin and 4-methoxysalicylic
aldehyde were detected by FAAS and electric
heating wet
digestion, and the data were analyzed by SPSS 19.0.
Results:
The seven
elements were varied widely in velamen and
stem bark. The variation
trends of Ca and periplocin, Fe and 4-methoxysalicylic
aldehyde in velamen were
similar, equally, those of Fe and 4-methoxysalicylic
aldehyde, Ca and Mg in
stem bark were
similar and like double peaks. Periplocin has highly significant positive correlation with Ca in velamen and significant negative correlation with Zn in
stem bark. To 4-methoxysalicylic
aldehyde, when in velamen, it has highly significant positive correlation with 4-methoxysalicylic
aldehyde and Fe in
stem bark, significant negative correlation with Mn in
stem bark, and significant positive correlation with Fe in velamen; when in
stem bark, it has highly significant positive correlation with Fe in both velamen and
stem bark.
Conclusion:
Ca, Zn, Fe, and Mn are dominant
elements of accumulation, the key factors for periplocin are Ca in velamen and Zn in
stem bark, and Mg in velamen is indierect acting factors; The key factors for 4-methoxysalicylic
aldehyde are Mn in
stem bark and Fe, Ca and Mg in
stem bark are indirect acting factors.