ABSTRACT
OBJECTIVEP To investigate
ultrasonic-assisted estraction(UAE) and response surface
methodology(RSM) for the extraction of asarinin from Asari Radix et Rhizoma(ARR).
METHODS:
The RSM was based on a three-level, four-variable Box-Behnken design (BBD). The independent variables were
ultrasonic time, liquid to solid ratio,
ultrasonic temperature, and
ultrasonic power, the dependent variable was extraction rate of asarinin, which was used to estimate the relationship between independent and dependent variables. Box-Behnken design and RSM were used to optimize the process of extraction. The prediction was carried out through comparing the observed and predicted values.
Antioxidant activity of the extract of ARR was determined by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH) and 2, 2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate(ABTS) radical scavenging assays
in vitro, and good correlation between extraction rate of asarinin and
antioxidant activity was observed.
RESULTS:
The results indicated that
ultrasonic time, liquid to solid ratio,
ultrasonic temperature, and
ultrasonic power had a significant effect on extraction rate of asarinin. Overall process intensification was achieved with
ultrasonic time of 56 min, liquid to solid ratio of 171 mL•g-1,
ultrasonic temperature of 52℃, and
ultrasonic power of 180 W by UAE
method. Under optimal conditions, the yield of asarinin was (1.55±0.32) mg•g-1 (n=3), which was in accordance with the predicted yield of 1.58 mg•g-1. The
IC50 values of the extract of ARR sample were 29.701 and 64.643 mg•mL-1, respectively. The
antioxidant results indicate that the extract of ARR has excellent
ability to scavenge
free radicals and
antioxidant capacity and is expected to be used as a natural
antioxidant in industrial applications.
CONCLUSION:
The extraction
technology is simple, reliable and highly predictive.The UAE
method is effective for extraction of asarinin from ARR.