ABSTRACT
OBJECTIVE:
To investigate the protective effects of aidi
injection on
cisplatin (CDDP)-induced acute nephrotoxicity in
rats.
METHODS:
Forty
male Sprague-Dawley rats were randomly divided into 5 groups, including the
control group, CDDP group, the low, medium and high
dose Aidi
injection group (5, 10, 15 mL·kg-1). Except the
rats in
control group, the
rats of other groups were injected with CDDP on the third day. Aidi
injection groups were administered to the
rats once daily for 7 consecutive days. The control and CDDP groups were given corresponding regime of saline. On the eighth day, determine the content of
serum creatinine (Scr) and
blood urea nitrogen (
BUN) in the
rat, detect the level of
superoxide dismutase (SOD),
glutathione reductase (GSH-Px) and
malondialdehyde (MDA) in the
kidney tissue; observe HE
staining slices of
rats and find the renal pathological changes.
RESULTS:
Compared with
control group, the levels of Scr,
BUN and MDA content were increased significantly in CDDP group, whereas the contents of SOD and GSH-Px were decreased significantly. There were serious, renal tubular
epithelial cell apoptosis and
inflammation in the
kidney tissue.
Kidney histopathology indicated that Aidi
injection alleviated CDDP-induced
tissue damage. The levels of Scr,
BUN were decreased significantly in the low
dose Aidi
injection group, whereas the activity of SOD and GSH-Px were increased significantly. The medium
dose of Aidi
injection could increase the level of SOD and GSH-Px. The high
dose of Aidi
injection could enhance the activity of GSH-Px, without statistically significant effect on other
indicators.
CONCLUSION:
The results indicate that the low and middle
dose of Aidi
injection might protect the renal
tissue from nephrotoxicity induced by
cisplatin through anti-
oxidation.