ABSTRACT
Objective:
To explore the application value of two-dimensional
shear wave elastography (2D-SWE) in
detection of
acute liver failure (ALF) and evaluation on prophylactic
drug use in
rats.
Methods:
Totally of 28
male SD
rats were divided into four groups, i.e.
control group (n=8), model group (n=8),
lactulose group (n=6) and Medilac-s group (n=6). From the 1st to the 8th day of the experiment,
rats in Medilac-s group were treated with intragastric
administration of Medilac-s after
dilution, in
lactulose group were given
lactulose, while in
control group and model group were given the same amount of
normal saline. On the 6th and 7th day of the experiment,
rats in model group,
lactulose group and Medilac-s group were intraperitoneally injected with
thioacetamide, while in
control group were injected with
normal saline. On the 8th day of the experiment, 2D-SWE was used for
liver stiffness measurement (LSM) in all 4 groups.
ROC curve was constructed, and the most accurate LSM value was selected according to
AUC used for inter-group comparison. On the 9th day of the experiment, the
liver of
rats were taken for pathological examination.
Results:
The main pathological manifestations in model group were
inflammation and
necrosis, in Medilac-s group and
lactulose group were
inflammation and
necrosis less than in model group, whereas normal
liver tissue was found in
control group. Among
ROC curves constructed by LSM value of the model group, the diagnostic
efficiency of LSMmean was the best. Taken 8.44 kPa as the cut-off value, the
AUC,
sensitivity and specificity was 0.768, 100% and 62.50%, respectively. LSMmean in model group was significantly higher than that in the other 3 groups (all P<0.05).
Conclusion:
Liver stiffness increases in ALF
rats. 2D-SWE has application value for
diagnosis of ALF and evaluation after prophylactic
drug use.