ABSTRACT
Objective:
To investigate the chemical constituents from the ethyl
acetate extract of 95%
ethanol extract from
Ajuga ovalifolia var. calantha.
Method:
A. ovalifolia var. calantha was percolated with 95%
ethanol，and the percolate was concentrated under reduced
pressure to obtain the extract. The extract was then dispersed with
water and extracted with
petroleum ether to obtain the
petroleum ether extract fraction and
water layer. Then ethyl
acetate was used to extract the
water layer to obtain the ethyl
acetate extract fraction. The Compounds from the ethyl
acetate extract fraction were isolated and purified by
silica gel，Sephadex LH-20，MCI column，ODS column
chromatography and semi-preparative
high performance liquid chromatography. Their structures were elucidated by interpretation of NMR，ESI-MS and other spectral evidence.
Result:
17 Compounds were isolated and elucidated as bakkenolide-E（1），loliotide（2），isololiotide（3），（E）-linalool-1-oic
acid（4），umbelliferone（5），phillygenin（6），1-（4-hydroxyphenyl）-
ethanol（7），（2E）-8-hydroxy-2，6-dimethyl-2-octenoic
acid（8），1H-indole-3-
carboxylic acid（9），ajugarin I（10），ajugalactone（11），
luteolin（12），
20-hydroxyecdysone-2-
acetate（13），benzyl-4'-hydroxy-benzoyl-3'-O-β-D-glucopyranoside（14），harpagide（15），6-deoxy-8-acetylharpagide（16），and acteoside（17）.
Conclusion:
Compounds 1，3-4，6-9，14 were isolated from the
plants of
Ajuga for the first
time，and compounds 2，5，10，13，15-16 were isolated from this
plant for the first
time.
