ABSTRACT
Objective:
To improve the
quality control system in the
production of Jinshuibao
capsules, and to provide experimental basis for the follow-up
research and application of this preparation.
Method:
High performance liquid chromatography (
HPLC) was employed, the
analysis was performed on a Ultimate AQ-C18 column (4.6 mm×150 mm, 5 μm). The chromatographic conditions for the
determination of
adenosine,
guanosine and
uridine were as following mobile phase of
methanol-0.1%formic
acid aqueous
solution for gradient elution, the flow rate of 0.4 mL·min-1, column
temperature at 30 ℃, sample quantity of 10 μL,
detection wavelength at 260 nm. The chromatographic conditions for the
determination of
ergosterol were as follows mobile phase of
methanol-
water (98∶2), the flow rate of 1 mL·min-1, column
temperature at 25 ℃, sample quantity of 10 μL,
detection wavelength at 283 nm.
Result:
The main chromatographic peaks of fermented
Cordyceps powder samples in different
production stages showed little difference. The linear relationships of
adenosine,
guanosine and
uridine were good (R2 >0.999), their recoveries were 106.06%, 101.25%and 105.88%, respectively. The contents of
adenosine and
ergosterol in 20 batches of samples extracted from 2016 to 2018 were in line with the requirements in the 2015 edition of
Chinese Pharmacopoeia, the contents of
guanosine and
uridine were 0.97-1.36, 0.67-1.38 mg/
capsule, respectively.
Conclusion:
The quality of Jinshuibao
capsules in the market is stable. This
method can be used to detect the quality of Jinshuibao
capsules, and it is simple, stable and reliable.