Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 111-121, 2021.
Article
在 Zh
| WPRIM
| ID: wpr-879256
Responsible library:
WPRO
ABSTRACT
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
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