ABSTRACT
Objective:
To analyze the sequence characteristics,chromosomal
location,
gene structure,conserved motifs,phylogenetic evolution and differential
gene expressions of the <italic>
Cannabis sativa</italic> YABBY
transcription factor family,in order to provide a molecular basis for in-depth study of <italic>YABBY</italic>
gene function and theoretical support for the selection and
breeding of superior
hemp varieties.
Method:
The
bio-informatics method was used to identify and analyze the <italic>CsYABBY </italic>
gene family of the original
hemp seed plant. PlantTFDB,ExPASy,MEME,CELLO,PLANTCARE and other online websites and TBtools,MEGA,DNAMAN and other
software were used for prediction,visualization and
analysis.
Result:
<italic>C. sativa</italic> contains 6 <italic>YABBY</italic>
gene members distributed on 5
chromosomes,in which 5 members are localized in the nucleus and 1 in extracellular, they consist of 185-235
amino acids, and the
isoelectric point is between 5.05 and 9.34, the
molecular weight is between 20 582.45-26 282.7 Da. All of CsYABBY
proteins contain two conserved domains, namely
Zinc finger domain and YABBY domain. <italic>CsYABBY</italic>
genes have multiple cis-acting
elements,and their expressions differ in different
tissues and cultivars.
Conclusion:
The expressions of CsYABBY may be affected by
hormones and externally environmental factors. <italic>CsYABBY</italic>
gene expressions are
tissue-specific. In addition,YABBY
transcription factor family may
play an important
role in regulating the development of <italic>C. sativa</italic>
female flowers,and subfamilies YAB1 and YAB5 may be involved in the synthesis of
cannabinoids.