ABSTRACT
Objective:
To explore an ideal
method for establishing a
mouse model of chronic
atrophic gastritis (CAG).
Methods:
CAG
mouse models were established with five different modeling
methods ( N-methyl- N′-nitro- N-nitrosoguanide (
MNNG),
sodium salicylate,
sodium deoxycholate,
Helicobacter pylori infection, and combinations of them) in BALB/c and C57
mice. The effect of each modeling
method was evaluated by histological
observation of
gastric mucosa,
plasma biochemical
parameters, inflammatory response score, and the expression of anti-inflammatory factors.
Results:
The results of histological
observation of
gastric mucosa showed that all of the 5
methods could successfully establish CAG
mouse models. In BALB/c
mice, compared with the healthy
control group, significant features of CAG accompanied with intestinal
metaplasia was found in the model group established by combination of
MNNG-free
drinking, 2%
sodium salicylate and 20 mmol
sodium deoxycholate. From the results of serological
detection, compared with the normal
control group, the
mRNA expression levels of related anti-inflammatory factors
interleukin-2,
interleukin-10,
interleukin-13 and
growth differentiation factor-15 of each model group decreased, which indicated that the
mice of each CAG model group had different degrees of
inflammation. The results of
plasma biochemical
parameters indicated that
plasma gastrin of each group decreased and the ratio of
pepsinogen Ⅰ and
pepsinogen Ⅱ significantly dropped. The above results demonstrated that in BLAB/c
mice,
MNNG-free
drinking, 2%
sodium salicylate and 20 mmol
sodium deoxycholate was better than other four modeling
methods. For C57
mice, it was also found that simple chemical
drug mutagenesis and
Helicobacter pylori replication
method both could successfully establish CAG models. No matter from pathological
observation, relative expression of anti-inflammatory factors and
analysis of
plasma biochemical
parameters, the effects of combination of the two
methods was better.
Conclusion:
The CAG
mouse model established by
MNNG-free
drinking, 2%
sodium salicylate and 20 mmol
sodium deoxycholate can provide a certain reference for the establishment and application of
mouse model in CAG experiments in the
future for pharmacological
research.