ABSTRACT
Objective:
To analyze the
epidemic characteristics and
virus gene sequence of
hemorrhagic fever with renal syndrome (
HFRS) in an industrial park in Daishan County, Zhejiang Province, and to provide clues and basis for local
HFRS prevention and control.
Methods:
According to the case
questionnaire in the "National
Surveillance Program for
Hemorrhagic Fever with Renal Syndrome", general and
epidemiological investigation of
HFRS cases was carried out in the
epidemic-related industrial park.
Serum samples of the cases, people and host
animals in the same living
environment were collected for
hantavirus antibody or
nucleic acid detection, the M, S
gene amplification and
sequence determination. MEGAX 10.1.8
software was used to construct the
phylogenetic tree of M and S
genes for
virus genotyping and evolutionary
analysis.
Results:
A total of 3 confirmed cases of
HFRS were reported. They were all
workers in the
epidemic-related industrial park,
male,
who lived in the park for more than half a year and had no
history of
HFRS vaccination. There were no
rodent-proof facilities in the industrial park's dormitories and canteens, and the living items were placed in a disorderly manner, the
rodents and its excrement could be seen; a total of 38 host
animals were captured in the same living
environment with cases, all of which were
Rattus norvegicus. The 3 reported cases of
HFRS were all mild, with atypical clinical manifestations in the early stage of onset, mainly
fever and
fatigue. The
serum specific
antibodies of
hantavirus IgG and
IgM were positive (3/3), and the
antibodies of people in the same living
environment were negative (100.0%, 100/100). The
serum samples of 2 reported cases of
HFRS and 4
Rattus norvegicus were positive for
nucleic acid, all of which were SEOV type
hantavirus. The M
gene segment homology of 6 positive
serum samples was 100.0%, which was closely related to Rod/2012/QHD/4/Gc isolated from Hebei and RuianRn180 isolated from Ruian Zhejiang Province; the homology of S
gene segment was 99.6% to 99.8%, which was closely related to JiangxiXinjianRn-09-2011, a
strain isolated from Jiangxi Province.
Conclusions:
The
HFRS epidemic in the industrial park is caused by the
transmission of SEOV type
hantavirus to
humans via
Rattus norvegicus; poor living
environment, poor
hygiene habits of
personnel and lack of
vaccination are all related to the
incidence of
HFRS; the main
epidemic strains shows high homology and geographical aggregation.