ABSTRACT
Objective:
To investigate the mechanism of
urokinase on inflammatory substances and
thrombomodulin (TM) in
deep vein thrombosis (DVT)
rats.
Methods:
A
rat model of
deep vein thrombosis was established. Thirty
rats were randomly divided into sham group, DVT group and UK (
urokinase) group. The
rat model of
deep venous thrombosis was established in DVT group and UK group. One day after operation,
urokinase (20 000 U/kg) was injected into caudal
vein in UK group once a day for 14 days; Sham group and DVT group were given the same volume of
normal saline. The wet weight and the ratio of wet weight/length of
thrombus were compared among the three groups; HE
staining was used to detect the pathological changes of
thrombus in the three groups; The
plasma inflammatory factors
interleukin-8 (
IL-8) and
tumor necrosis factor α (TNF-α) were detected by
enzyme-linked immunosorbent assay (
ELISA). The
mRNA expression of TM in the three groups was detected by real-
time fluorescence quantitativepolymerase
chain reaction (qRT-
PCR).
Results:
Compared with sham group,
thrombosis was found in DVT group. The wet weight and wet weight/length ratio of
thrombus in DVT group were significantly higher than those in sham group ( P<0.05); After
urokinase intervention, the wet weight of
thrombus in UK group was significantly lower than that in DVT group, and the wet weight/length ratio of
thrombus was also significantly lower than that in DVT group ( P<0.05). Compared with sham group, DVT group had obvious
thrombosis,
granulation tissue covered around the
tissue, obvious adhesion between
blood vessels and tube wall, a large number of inflammatory
cell infiltration around venous
tissue and obvious destruction of valve structure; After
urokinase intervention, the
thrombus tissue of UK group was significantly improved. Compared with sham group, the concentration of
IL-8 , TNF-α and sTM in DVT group were significantly increased ( P<0.05). After
urokinase intervention, the
IL-8, TNF-α and sTM concentration in UK group were significantly lower than those in DVT group ( P<0.05). qRT-
PCR results showed that TM
mRNA expression in DVT group was significantly higher than that in sham group ( P<0.05). The TM
mRNA expression in UK group was significantly lower than that in DVT group ( P<0.05).
Conclusions:
Urokinase can inhibit the inflammatory factors and the expression of
thrombomodulin in DVT.