ABSTRACT
Objective:
To observe effect of
alprostadil combined with
Diammonium glycyrrhizinate on renal interstitial
fibrosis in SD
rats.
Methods:
A total of 75 SD
rats were randomly divided into A, B, C, D, E groups with 15 in each group.
Rats in group A served as the
control group received just only but
tissue separation without modeling operation, while model of unilateral
ureteral obstruction (UUO) was established in B, C, D, E groups.
Rats in A, B group were given saline
lavage placebo
treatment, while
rats in C, D, E groups were given
diammonium glycyrrhizinate and
alprostadil injection. Five
rats were sacrificed 1, 2, 3 weeks after modeling,
serum creatinine level of femoral venous
blood was determined.
Transforming growth factor - β1 (TGF - β1) and concentration of
connective tissue growth factor (CTGF) were also detected by using
ELISA. Line renal interstitial
tissue was taken after HE
staining, renal interstitial TGF - β1 and CTGF expression were detected by using immunohistochemical
method.
Results:
Serum creatinine levels of B, C, D, E group at different
time points in were significantly higher than that of group A (. P0.05); while
serum and
kidney tissue TGF - β1, concentration of CREA, expression of
rats in B, C, D, E groups showed a gradual increasing trend over
time. TGF - β1 and CREF of Group B in
serum and
kidney tissues at each
time point were significantly higher than that of the other groups (. P<0.05). TGF - β1 and CREF of Group E in
serum and
kidney tissues at each
time point were significantly lower than that of B, C, D group at all
time points in
serum and
kidney tissues (. P<0.05).
Conclusions:
Alprostadil combined with
diammonium glycyrrhizinate can significantly lower the expression of TGF - β1 and CTGF in
serum and
tissues of SD
rat with renal interstitial
fibrosis, thus inhibit
rat renal interstitial
fibrosis process. It has synergy protective effect.