Exosomes derived from Nr-CWS pretreated MSCs facilitate diabetic wound healing by promoting angiogenesis via the circIARS1/miR-4782-5p/VEGFA axis / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 172-184, 2023.
Article
在 En
| WPRIM
| ID: wpr-971676
Responsible library:
WPRO
ABSTRACT
Mesenchymal stem cell (MSC)-derived exosomes (Exos) were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect. MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes. Hence, this study was designed to explore whether exosomes derived from human umbilical cord MSCs (hucMSCs) preconditioned with Nocardia rubra cell wall skeleton (Nr-CWS) exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms. The results showed that Nr-CWS-Exos facilitated the proliferation, migration and tube formation of endothelial cells in vitro. In vivo, Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos. Furthermore, the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos. CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cellsvia the miR-4782-5p/VEGFA axis. Taken together, those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.
Key words
全文:
1
索引:
WPRIM
主要主题:
Wound Healing
/
Cell Wall Skeleton
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Neovascularization, Physiologic
/
MicroRNAs
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Endothelial Cells
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Vascular Endothelial Growth Factor A
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Diabetes Mellitus
/
Exosomes
限制:
Humans
语言:
En
期刊:
Chinese Journal of Natural Medicines (English Ed.)
年:
2023
类型:
Article