ABSTRACT
Objective:
To explore and analyze the value of
detection of peripheral
blood miR-194 combined with fecal miR-143 in the clinical
screening of
colorectal cancer.
Methods:
A total of 83
patients diagnosed with
colorectal cancer by pathological
tissue admitted to Huangshi
Hospital of
Traditional Chinese Medicine of Hubei Province from October 2019 to October 2020 were selected as the
observation group, and 50
healthy volunteers who underwent
physical examinations during the same period were selected as the
control group. The levels of miR-194 in peripheral
blood and miR-143 in
feces were detected by
fluorescence quantitative
PCR. The level difference between the two groups and their correlations with clinicopathological
parameters of
patients with
colorectal cancer were analyzed.
Receiver operating characteristic (ROC) curve was drawn based on peripheral
blood miR-194 and fecal miR-143 to evaluate their value for clinical
screening of
colorectal cancer.
Results:
The level of miR-194 in peripheral
blood of the
observation group was significantly higher than that of the
control group (1.91±0.34 vs. 0.76±0.23) , while the level of fecal miR-143 in the
observation group being significantly lower than that of the
control group (1.85±0.43 vs. 2.48±0.62) , with statistically significant differences ( t=21.16, P<0.001; t=6.91, P<0.001) . Age of
patients with
colorectal cancer ( t=0.83, P=0.408; t=1.17, P=0.244) , TNM stage ( t=1.03, P=0.307; t=0.11, P=0.909) ,
lymphatic metastasis ( t=0.37, P=0.711; t=1.85, P=0.068) , distant
metastasis ( t=0.41, P=0.683; t=1.72, P=0.089) were not correlated with the levels of peripheral
blood miR-194 and fecal miR-143. When the cut-off value of miR-194 in peripheral
blood was 1.82, the area under the
ROC curve for the
diagnosis of
colorectal cancer was 0.76, and the diagnostic
sensitivity and specificity were 79.38% and 74.29%, respectively. When the cut-off value of fecal miR-143 was 2.16, the area under the
ROC curve for the
diagnosis of
colorectal cancer was 0.71. At this
time, the diagnostic
sensitivity and specificity were 76.54% and 73.61%, respectively. The area under
ROC curve of combined
detection for
colorectal cancer was 0.81, and the diagnostic
sensitivity and specificity were 83.46% and 75.43%, respectively.
Conclusion:
Peripheral
blood miR-194 is highly expressed in
colorectal cancer patients, and fecal miR-143 is low in
colorectal cancer patients. The combined
detection of the two has a high
sensitivity for
early diagnosis of
colorectal cancer, which can provide important reference basis for
early diagnosis of
colorectal cancer and has high clinical application value.