ABSTRACT
Objective:
In order to investigate the
genetic diversity of mitochondrial
cytochrome b (Cytb)
gene of
Taenia asiatica ( T. asiatica) in Dali Bai Autonomous Prefecture (Dali Prefecture), Yunnan Province.
Methods:
From May 2019 to August 2021, a total of 131 samples of
Taenia were collected from
patients admitted to the Dali Prefecture Institute of
Schistosomiasis Control, involving five locations (i.e., five groups), including Dali City (58 samples), Weishan Yi and Hui Autonomous County (Weishan County, 14 samples), Midu County (18 samples), Yangbi Yi Autonomous County (Yangbi County, 24 samples) and Eryuan County (17 samples). Primers were designed based on mitochondrial Cytb
gene sequence, and part of the Cytb
gene sequence was amplified by
PCR, then sequenced and homology comparisons were performed. MEGA 7.0 and DNASP 5.10.01 were used to analyze the measured sequence, and data such as
base composition, genetic distance,
genetic diversity parameters, genetic differentiation index and
gene flow were obtained.
Results:
The amplified fragments of Cytb
gene in 131 samples of
Taenia were 235 bp. After homology comparisons, they were all T. asiatica. The average contents of A, T, G and C bases were 23.8%, 42.3%, 24.0% and 9.9%, respectively. Of the 131 samples of T. asiatica, 12
haplotypes were defined. The
haplotype diversity and
nucleic acid diversity were 0.295 9 and 0.006 0, respectively. The ranges of genetic differentiation index and
gene flow among the five groups were-0.053 00 to 0.050 40 and 4.710 31 to 162.087 66, respectively. The genetic distance between the five groups ranged from 0.003 5 to 0.009 0, of which the genetic distance between Midu County and Weishan County was the largest, and the genetic distance between Dali City and Yangbi County was the smallest.
Conclusions:
The mitochondrial Cytb
gene of T. asiatica in Dali Prefecture has rich
genetic diversity. There is frequent
gene exchange among the five groups, and no significant genetic differentiation has been formed.